The LSR Fortessa has a control panel with three speeds: Low (~12 µl/min), Medium (~35 µl/min) and High (~60 µl/min). There is also a sample fine rate knob to the left. The LSR-Fortessa can handle over 10,000 events a second without loss of accuracy. Sample Injection Port (SIP):(Tube support arm) SIP has two positions.

BD Biosciences reserves the right to change its products and services at any and optical filters to direct the resulting light signals to the appropriate detectors.

650. 785. BD. 750. 780/60. A. APC-Cy7. BD LSR Fortessa.

The BD LSRFortessa X-20 uses configurable polygon-shaped optical pathways that use signal reflection to maximize signal detection. In 1897, Maxwell Becton and Fairleigh Dickinson founded Becton, Dickinson and Company with a vision to improve outcomes for patients. With more than a century of experience and our global reach, BD leads in patient and healthcare worker safety and the technologies that enable medical research and clinical laboratories. BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 635LP PE-Cy5, 7AAD, mPlum C 610/20 600LP PE-Texas Red, PE-CF594 We set up our configuration such that the parameter names specify the laser, detector and filter, 642 C 675/20, etc. as the defaults and added a couple of common filters for corresponding detectors for folks that like to tighten up filters for some panels, like a 712/20 for A700 or BV711, 426/27 for BV421, etc. BD have been highly supportive throughout the process, even bringing the director of SORP to look at the instrument.

BD LSRFortessa™ Cell Analyzer; Special Order BD LSRFortessa™ Cell Analyzer Product Information Sheets. BD™ Optical Filters: Optimal detection of BD Horizon™ Brilliant Violet™ Polymer Conjugates Quick Reference Guides.

BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 635LP PE-Cy5, 7AAD, mPlum C 610/20 600LP PE-Texas Red, PE-CF594

BRIGHTEST. Brilliant Violet™ 421. 450/50.

Alexa Fluor 405, BD Horizon V450, BFP, BV421, Calcein Violet, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet,. eFluor 450, Fixable Violet Dead Cell

The 5 Laser LSR Fortessa (“5L”) is located in CNY149, room.

Silence alarm by clicking bdbiosciences.com 23-11093-00 Rev. A 3/2010 For Research Use Only Becton, Dickinson and Company BD Biosciences San Jose, CA 95131 Tel 877.232.8995 Fax 800.325.9637 BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3.
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BD Fortessa X-20 step to 10 minutes. BD LSR Fortessa The FCF staff can provide sterile re-usable filters when needed.

4) Start BD FACSDiva software. Group User and Password 5) If working with bacteria, open the black nob in front of the FSC. 6) Open the optical filter compartment (on the left side of the Fortessa and two small ports in front) to make sure that the filters necessary for detecting your fluorophores are in place.
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arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options. Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC)

FITC/BB5815/VioBright FITC/Alexa488. 695/40. PerCP*, PerCP-Cy5.5™, PE-Cy5™ (PC5), PE-Cy5.5™**, PerCP-eFluor® 710, The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry simultaneously with a defined set of optical filters that meet or exceed the The cytometer configuration defines which filter and mirror are installed at each detector.

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BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes. Table of lasers and filters available: Laser Filters

Fluorescent. Proteins. Proliferation BD Fortessa Configuration. KCRB. Room 4161. BD LSRFortessa SORP cell analyzer Lasers: Excitation.